Prussian blue (PB) has unique advantages and is widely used in the field of colorimetric detection. On the one hand, PB can convert light energy into heat energy under near-infrared light (NIR) irradiation, which is an excellent photothermal transducer and suitable for photothermal therapy (PTT) in the biomedical field. On the other hand, PB has excellent peroxidase-like (POD) activity, which can catalyze the oxidation of the color developer 3,3′,5,5′-tetramethylbenzidine (TMB) to produce the blue oxide oxTMB in the presence of H2O2.
Prussian blue nanoparticles (PBNPs), which combine the properties of nanomaterials and PBs, are increasingly used in biomedical applications. Studies have shown that due to their porous structure and easy surface modification, PBNPs are able to carry drug molecules and precisely release drugs in response to stimuli from the external environment, improving therapeutic efficiency. In the field of medical imaging, PBNPs can be used as imaging contrast agents to enhance image display due to their strong absorption in the near-infrared (NIR) region, high photothermal conversion efficiency and paramagnetism, as well as nano-enzymatic properties, which can catalyze the removal of excess reactive oxygen species (ROS) in the body, which can be used for photodynamic therapy of tumors and the treatment of ROS-related diseases.
Materials and Reagents
PB solution (0.2 mg/mL)
PBS buffer
Protease inhibitors
TMB
H2O2
Horseradish Peroxidase-Biotin (HRP-Bio) solution (1 mg/mL)
PD-L1 aptamer solution (10 µmol/mL)
N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) solution (0.5 mol/L)
N-Hydroxysuccinimide (NHS) solution (0.5 mol/L)
FITC-Avidin (FITC-Avi) solution (0.5 mg/mL)
Equipment
Centrifuge, Sonicator, -80℃ freezer, Shaking incubator, UV-Vis spectrophotometer